Benjamin Pardo

This study shows that Slx4 and Fun30/SMARCAD1 locally dampen the S-phase checkpoint to promote replication fork protection and completion in response to Top1-DNA crosslinks.

This paper introduces a CRISPR-Cas9-based system to induce controlled DNA double strand breaks (DSBs) in yeast and studies the behavior of the Tel1 kinase in response to these breaks.

The paper induces unscheduled DNA replication in G1-phase budding yeast, showing it leads to genome instability through head-to-tail replication fork collisions during subsequent S-phase.

The paper shows that Mus81 is essential for resolving recombination intermediates during replication restart in G2/M phase, independent of DNA supercoiling and crosslinks.

qDSB-Seq quantifies absolute DNA double-strand break frequencies and their genomic coordinates across samples and organisms.