The paper determines the cryo-EM structure of the AMBRA1-DDB1 complex bound to cyclin D1, revealing the molecular mechanism of cyclin D1 recognition and its regulation by phosphorylation.

Science Advances · May 23, 2025Peer-reviewedBiochemistry

Structural basis for membrane remodelling by the AP5:SPG11-SPG15 complex

Xinyi Mai, Yang Wang, Xi Wang, Ming Liu, Fei Teng, Zheng Liu, Ming-Yuan Su, Goran Stjepanović

This study determines the structure of the AP5:SPG11-SPG15 complex and shows it binds PI3P, senses membrane curvature, and drives membrane remodeling in vitro.

Nature Structural & Molecular Biology · Apr 2, 2025Peer-reviewedBiochemistry

Enhanced Recognition of a Herbal Compound Epiberberine by a DNA Quadruplex-Duplex Structure

Xuan Zhan, Liping Deng, Yun Lian, Zhiyu Shu, Yunong Xu, Xinyi Mai, Manchugondanahalli S. Krishna, Rongguang Lu, Anni Wang, Shiyao Bai, Fangyu Zhou, Chi Xiong, Yingyi Xu, Jie Ni, J. Jeya Vandana, , Gang Chen

This paper investigates how epiberberine binds to a DNA quadruplex structure and shows how modifying the DNA structure enhances binding affinity, leading to a new method for extracting the compound from plant extracts.

Analytical Chemistry · Aug 2, 2024Peer-reviewedBiochemistry

A Conformation-specific Nanobody Targeting the NMN-activated State of SARM1

Yun Nan Hou, Yang Cai, Wan Hua Li, Wei Ming He, Zhi Ying Zhao, Wen Jie Zhu, Qiang Wang, Jun Liu, Hon Cheung Lee, Goran Stjepanović, Hongmin Zhang, Yong Juan Zhao

The paper develops a nanobody that specifically targets the NMN-activated state of SARM1, enabling structural analysis and functional studies of this conformation.

bioRxiv · Mar 25, 2022 · PreprintMolecular Biology