Christine L. Mummery

STRAIGHT-IN Dual: a platform for dual, single-copyintegrations of DNA payloads and gene circuits into human induced pluripotent stem cells

STRAIGHT-IN Dual enables efficient, single-copy integration of two DNA payloads into hiPSCs, improving genetic engineering for synthetic biology applications.

Gene syntax defines supercoiling-mediated transcriptional feedback

The paper shows that gene syntax influences supercoiling-mediated transcriptional feedback, affecting adjacent gene expression in human cells.

A simplified co-culture reveals altered cardiotoxic responses to doxorubicin in hPSC-derived cardiomyocytes in the presence of endothelial cells

This study shows that endothelial cells enhance doxorubicin-induced cardiotoxicity in co-cultures with cardiomyocytes, challenging the focus on cardiomyocytes alone.

Industrialization of three-dimensional hiPSC-cardiac microtissues for high-throughput cardiac safety and drug discovery screening

This study develops scalable 3D cardiac microtissues for high-throughput drug screening, showing reproducible drug responses and identifying 17 drugs that rescue arrhythmic phenotypes.

Controlled Induction of ALK1 in ACVRL1-null hiPSC-derived Endothelial Cells Provides Insight into Organ-Restricted AVMs in HHT

The paper develops a tunable in vitro model to study ALK1's role in HHT by inducing ALK1 in ACVRL1-null hiPSC-derived endothelial cells, showing threshold-dependent restoration of endothelial function.

Engineered microvasculature using maskless photolithography and on-chip hydrogel patterning: a facile approach

This paper describes a method to create microvasculature models using photolithography and hydrogel patterning, enabling rapid and tunable fabrication of perfusable vascular structures from stem cell-derived endothelial cells.

ETV2 upregulation marks the specification of early cardiomyocytes and endothelial cells during co-differentiation

The paper identifies ETV2 dynamics in hiPSC-derived cardiac lineages using single-cell RNA-seq and a fluorescent reporter, revealing distinct subpopulations for endothelial and cardiomyocyte specification.

Tissue microenvironment dictates the state of human induced pluripotent stem cell-derived endothelial cells of distinct developmental origin in 3D cardiac microtissues