David J. Stillman

The paper investigates a Tup1 mutant in yeast, finding it acts as a coactivator rather than a corepressor, challenging the classic model of gene repression.

The paper shows that Ash1 repressor binds to the HO promoter in yeast, requiring nucleosome eviction by Swi5 for access, and that this process is linked to repression and promoter resetting.

The paper shows that the Mediator Tail module can be recruited independently to the HO promoter in yeast, suggesting the core Mediator complex limits coactivator recruitment and gene activation.

The paper identifies negative regulators that restrict SWI/SNF chromatin remodeler recruitment to the HO promoter in yeast through genetic screens and ChIP assays.

The paper investigates how FACT activity and H3-K56 acetylation influence chromatin architecture and transcription in yeast, finding they optimize transcription frequency and restore chromatin integrity independently of ongoing transcription.