Nikolaos-Nikiforos Giakoumakis

Multi-Site Reproducibility Study of 3D High-Content Analysis with Dual-View Oblique Plane Microscopy

This study assesses the reproducibility of a 3D spheroid-based assay across four institutes using dual-view oblique plane microscopy and finds high consistency in treatment effects with low technical noise.

McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylation

This study identifies McIdas as a regulator of centriole numbers through PLK4-dependent phosphorylation, linking its role in cell cycle control to centriole duplication.

In silico analysis of DNA re-replication across a complete genome reveals cell-to-cell heterogeneity and genome plasticity

The paper presents a stochastic hybrid model of DNA re-replication in fission yeast, showing cell-to-cell heterogeneity and genome plasticity through simulations and experimental validation.

Direct binding of Cdt2 to PCNA is important for targeting the CRL4Cdt2 E3 ligase activity to Cdt1

The paper shows that Cdt2 binds PCNA via a PIP box, enhancing CRL4Cdt2 activity and Cdt1 ubiquitination, suggesting a new mechanism for substrate targeting.