This paper introduces sdAb(mNG), a high-affinity nanobody that enables versatile applications for mNG-tagged proteins, including imaging, immunoprecipitation, and in vivo manipulation.

bioRxiv · Jul 1, 2026 · PreprintBiochemistry

N-acetyl-L-leucine (Levacetylleucine) normalizes Transcription Factor EB (TFEB) activity by stereospecific bidirectional modulation

Lianne C. Davis, Rebecca Braine, Grant C. Churchill, Mallory Factor, Taylor Fields, Marc C. Patterson, Frances M. Platt, Michael Leo Strupp, Antony Galione

This paper demonstrates that levacetylleucine modulates TFEB activity in a bidirectional manner, normalizing lysosomal function in both wild-type and NPC1 knockout cells.

bioRxiv · Dec 2, 2025 · PreprintPharmacology & Toxicology

Alternative Splicing Directs PMCA2 to Lysosomes and is Linked to Neurodegeneration

María E. Fernández‐Suárez, R. Bush, Jonathan Brenton, Gustavo J.S. Pereira, Melissa Grant‐Peters, Regina Hertfelder Reynolds, Daniëlle te Vruchte, Dawn Shepherd, Y. Weng, Ernesto Artaza-Fernandez, Paweł Lis, Luis Sánchez‐Pulido, Anthony John Morgan, Lianne C. Davis, Diego Gómez‐Coronado, , Frances M. Platt

The paper identifies a splice variant-dependent localization of PMCA2 to lysosomes and links it to neurodegenerative diseases.

bioRxiv · Oct 1, 2025 · PreprintCell Biology

Stereospecific rapid activation of Transcription Factor EB (TFEB) by Levacetylleucine (NALL)

Lianne C. Davis, Rebecca Brain, Grant C. Churchill, Mallory Factor, Taylor Fields, Frances M. Platt, Marc C. Patterson, Michael Leo Strupp, Antony Galione

This paper shows that NALL activates TFEB, enhancing lysosomal and autophagic function, which may explain its therapeutic effects in neurodegenerative diseases.

bioRxiv · Jun 6, 2025 · PreprintPharmacology & Toxicology