The paper identifies a specific Kv2/Kv6.4 channel subtype in spinal motor neurons localized at C-bouton synapses, showing dependence on Kv2 subunits for expression and clustering.

bioRxiv · Jun 4, 2025 · PreprintNeuroscience

Neurosphere culture derived from aged hippocampal dentate gyrus

Olga Vafaeva, Poommaree Namchaiw, Karl D. Murray, Elva Dı́az, Hwai-Jong Cheng

This paper introduces a novel neurosphere culture system using NPCs from adult and aged mice to study adult-born NPCs and their role in hippocampal function and aging.

STAR Protocols · Mar 1, 2025Peer-reviewedNeuroscience

Electrically silent KvS subunits associate with native Kv2 channels in brain and impact diverse properties of channel function

Michael Ferns, Deborah A. van der List, Nicholas C. Vierra, Taylor A Lacey, Karl D. Murray, Michael Kirmiz, Robert G. Stewart, Jon T. Sack, James S. Trimmer

The paper identifies Kv5.1 as a component of native Kv2.1 channels in mouse brain and shows it affects channel function and clustering.

Journal of Neuroscience · Feb 11, 2025Peer-reviewedNeuroscience

Developing a Toolbox of Antibodies Validated for Array Tomography-Based Imaging of Brain Synapses

Kristina D. Micheva, Belvin Gong, Forrest Collman, Richard J. Weinberg, Stephen J Smith, James S. Trimmer, Karl D. Murray

The paper develops a screening strategy and database of antibodies validated for array tomography-based imaging of brain synapses, improving antibody selection for this technique.

eNeuro · Nov 9, 2023Peer-reviewedNeuroscience

NeuroMabSeq: high volume acquisition, processing, and curation of hybridoma sequences and their use in generating recombinant monoclonal antibodies and scFvs for neuroscience research

Keith G. Mitchell, Belvin Gong, Samuel S. Hunter, Diana Burkart‐Waco, Clara E. Gavira-O’Neill, Kayla M. Templeton, Madeline E. Goethel, Malgorzata Bzymek, Leah M. MacNiven, Karl D. Murray, Matthew Lee Settles, Lutz Froenicke, James S. Trimmer

NeuroMabSeq sequences hybridoma cells to generate recombinant monoclonal antibodies and scFvs for neuroscience research, making them publicly available.

bioRxiv · Jun 28, 2023 · PreprintNeuroscience

Alterations in retrotransposition, synaptic connectivity, and myelination implicated by transcriptomic changes following maternal immune activation in non-human primates

Nicholas F. Page, Michael J. Gandal, Myka L. Estes, Scott Cameron, Jessie E. Buth, Sepideh Parhami, Gokul Ramaswami, Karl D. Murray, David G. Amaral, Judy Van de Water, Cynthia Mills Schumann, Cameron S. Carter, Melissa D. Bauman, A. Kimberley McAllister, Daniel H. Geschwind

This study identifies transcriptomic changes in non-human primate brains exposed to maternal immune activation, revealing alterations in retrotransposition, synaptic connectivity, and myelination.

Biological Psychiatry · Nov 2, 2020Peer-reviewedNeuroscience

A toolbox of nanobodies developed and validated for diverse neuroscience research applications

Jie-Xian Dong, Yongam Lee, Michael Kirmiz, Stephanie Palacio, Camelia Dumitras, Claudia M. Moreno, Richard C. Sando, Luis Fernando Santana, Thomas Christian Südhof, Belvin Gong, Karl D. Murray, James S. Trimmer

The paper develops and validates a set of nanobodies for targeting proteins in brain neurons, providing a toolbox for visualization and modulation of subcellular sites.

bioRxiv · May 9, 2019 · PreprintNeuroscience

A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain

Nicolas P. Andrews, Justin X. Boeckman, Colleen F. Manning, Joe Truong Nguyen, Hannah Bechtold, Camelia Dumitras, Belvin Gong, Kimberly Nguyen, Deborah A. van der List, Karl D. Murray, JoAnne Engebrecht, James S. Trimmer

The paper describes a method to generate and validate recombinant monoclonal antibodies for neuroscience research, enabling multiplex immunolabeling of brain tissue.

eLife · Jan 22, 2019Peer-reviewedNeuroscience