Aixin Zhang

Robust and accessible in situ sequencing with an open-source automated sequencer

The paper introduces Seq-o-Matic, an open-source automated in situ sequencer that reduces hands-on time and improves sequencing consistency across tissue sections.

Selective collateralization of transcriptomically distinct neurons organizes visual-stream output from the primary visual cortex

The paper maps gene expression and axonal projections of neurons in the mouse primary visual cortex to reveal how retinotopy and cell-type-specific collateralization organize visual-stream output.

Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing

This study uses barcoded rabies virus with single-cell and in situ sequencing to map synaptic connectivity in the mouse brain, distinguishing cortical cell types by their connectivity patterns.

Whole-cortex in situ sequencing reveals peripheral input-dependent cell type-defined area identity

The paper uses BARseq to analyze transcriptomic signatures in mouse cortex, showing that cell type composition defines area identity and is shaped by peripheral inputs.