Lei Jin

Comparative Study of BBB-Targeting AAV Capsids on CentralNervous System Delivery Efficiency

This study compares BBB-targeting AAV capsids for CNS delivery efficiency and safety, finding CNSRCV300 offers the best balance between brain targeting and biosafety.

Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing

This study uses barcoded rabies virus with single-cell and in situ sequencing to map synaptic connectivity in the mouse brain, distinguishing cortical cell types by their connectivity patterns.

"Self-inactivating" rabies viruses are susceptible to loss of their intended attenuating modification

The paper shows that a modified rabies virus intended for noncytotoxic neuronal tracing lost its modification due to mutations, leading to paradoxical results, and that the modified virus requires exogenous protease for efficient spread.

Single-deletion-mutant, third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency

This paper introduces third-generation rabies viral vectors with single deletions that enable more efficient, non-toxic retrograde targeting of projection neurons compared to previous generations.

Long-term labeling and imaging of synaptically-connected neuronal networks in vivo using nontoxic, double-deletion-mutant rabies viruses

This paper introduces a non-toxic rabies virus system for long-term in vivo imaging of neuronal networks, showing minimal toxicity and normal visual response properties over 10 weeks.

Monosynaptic tracing success depends critically on helper virus concentrations

The paper shows that helper virus concentrations significantly affect transsynaptic tracing efficiency and nonspecific labeling in monosynaptic tracing experiments.

High Current Optogenetic Channels For Stimulation And Inhibition Of Primary Rat Cortical Neurons

The paper demonstrates the use of ChR2-XXL and GtACR1 in primary rat cortical neurons to manipulate neuronal firing patterns through high current optogenetic channels.