Shenqin Yao

3D-MAESTRO automates processing of large-scale volumetric brain microscopy data, enabling scalable and reproducible analysis of mouse brain structures.

The paper presents a barcoded multiplex strategy to screen enhancer AAVs using single-cell RNA sequencing and taxonomy mapping, but finds significant technical and biological noise complicates accurate expression pattern analysis.

This study shows that cell type-specific AADC expression in Parkinson's disease models reduces motor deficits and dyskinesia more effectively than broad targeting.

The paper reveals the external globus pallidus (GPe) as a basal ganglia output hub with distinct subpopulations that mediate specific behaviors through projection-specific circuits.

The paper develops a cross-species enhancer-AAV toolkit for targeting specific cell types in the basal ganglia across species, validated in mouse and macaque.

The paper analyzes multi-modal Patch-seq data from macaque striatum to reveal morphoelectric diversity and functional specialization of neuronal cell types, highlighting primate-specific features and differences from rodents.

The paper creates a cross-species spinal cord cell type atlas integrating data from mouse, macaque, and human, identifying conserved and species-specific features.

This study shows psilocybin alters large-scale cortical network connectivity in mice by strengthening inputs from perceptual and medial regions and weakening cortico-cortical loops, dependent on drug-evoked spiking activity.

This paper introduces CellTransformer, a self-supervised framework for detecting fine-grained spatial domains in mouse brain spatial transcriptomics data, achieving high consistency across large datasets.

The paper identifies enhancers for spinal motor neurons and develops AAV vectors to target these cells in rodents and macaques, enabling functional studies and potential therapeutic applications.

This study integrates molecular and morphological data to classify cortical neurons and map their connectomes, revealing organizational principles of brain-wide networks at the single-cell level.

The paper uses viral labeling and Patch-seq to define morpho-electric properties of GABAergic interneurons in human neocortex, revealing functional insights and reclassifying some SST neurons into PVALB subclass.

The paper develops AAV-based genetic tools to specifically target astrocytes and oligodendrocytes across species, revealing functional roles in reward-dependent acetylcholine responses.

This study uses barcoded rabies virus with single-cell and in situ sequencing to map synaptic connectivity in the mouse brain, distinguishing cortical cell types by their connectivity patterns.

This study maps the cell-type-specific afferent and efferent projections of the mouse claustrum using viral tracing and single-neuron reconstruction, revealing its widespread connections and regional specificity.

The paper maps brain-wide monosynaptic inputs to specific neuron classes in mouse visual cortex using an improved rabies virus tracing strategy and identifies connectivity patterns within and between visual areas.

The paper combines calcium imaging and spatial transcriptomics to study functional and molecular properties of neuronal subclasses in the somatosensory cortex during a tactile working memory task.

The paper provides a detailed cellular-resolution map of the mouse primary motor cortex, defining cell types and their connectivity.

The paper develops enhancer viruses and a transgenic mouse line to enable combinatorial labeling of specific neuronal subclasses in the mouse cortex and human slices.

The paper identifies enhancer elements that drive subclass-specific gene expression in neocortex across mouse, primate, and human species using comparative epigenetics and AAV-based functional assays.

The paper introduces a light-inducible recombinase system for targeted genomic modifications in neurons, enabling precise labeling and functional analysis of neural circuits in live mouse brains.

The paper maps the connectivity of VMHvlEsr1 neurons in mice, revealing distinct subpopulations with different collateralization patterns but similar inputs, suggesting a unique circuit architecture for social behavior control.

This study shows that oxytocin signaling in the medial amygdala is essential for male mice to discriminate female social cues.