Robert Tjian

This study reports cryo-EM structures of PRC2.1 complexes with different cofactors, revealing how they diversify function while maintaining conserved catalytic mechanisms.

The paper uses an arrayed CRISPR screen to identify ZBTB10 as a regulator of human trophoblast differentiation, showing its role in fusion and hormone secretion.

This study shows that increasing MyoD1 levels in myoblasts expands its genome binding and upregulates cell adhesion genes, leading to impaired fusion but enhanced myotube contraction.

This study identifies FOXA1 mutations in prostate cancer, showing they alter chromatin accessibility and promote tumorigenicity by reprogramming cell fate through interactions with androgen receptor and POU2F1.

This study uses PAPA-fSMT to investigate P-TEFb interactions and exchange in live cells, revealing dynamics of the 7SK ribonucleoprotein complex and its association with chromatin and BRD4.

This study uses PAPA to show that IDRs of TFs mediate chromatin binding and interactions, challenging classical models of TF specificity.

The paper identifies HR factors that inhibit dual AAV vector transduction and shows that inhibiting these factors improves transgene reconstitution.

The paper uses automated live-cell single-molecule tracking to study transcription factor dynamics in mouse enteroid monolayers, revealing contrasting diffusive behaviors that correlate with differentiation stages.

This study reanalyzes Brd2 depletion effects on chromatin compartments using Micro-C data and cooltools, showing persistent but reduced compartment strength changes.

This study identifies TFEB as a transcriptional regulator of syncytiotrophoblast differentiation by controlling Syncytin-1 and Syncytin-2 expression in BeWo cells.

The paper investigates how HIF-1 and HIF-2 subunits exhibit distinct binding dynamics and gene regulation despite sharing the same DNA binding specificity, revealing the role of intrinsically disordered regions in chromatin binding and target selectivity.

This study investigates how tuning low-complexity domain interactions in the oncogenic TF EWS/FLI1 affects transcription and oncogenic transformation in human cells.

The paper investigates how enhancer-promoter interactions and transcription are affected by acute loss of CTCF, cohesin, WAPL, and YY1, finding minimal effects on E-P interactions and gene expression despite 3D genome perturbations.

The paper investigates how transcriptional regulatory elements influence the 3D organization of microTADs in mouse stem cells.

This study uses super-resolution imaging to reveal the 3D structure and organizing mechanism of accessible chromatin domains.

The paper shows that herpesvirus creates replication compartments that recruit RNA Polymerase II through DNA interactions, not phase separation, leading to local protein accumulations.

The paper identifies an integrated gene repression mechanism in human cells through mRNA isoform toggling at the MDM2 locus, similar to yeast.

This study uses live-cell imaging to show MeCP2 has unique diffusion and chromatin binding kinetics in neurons, distinct from transcription factors and histones.

The paper shows TBP remains bound to promoters during mitosis, stabilizing RNA Polymerase II association and enabling transcriptional reactivation.

This study uses live-cell single-molecule imaging to show that TF low-complexity domains form dynamic, selective interaction hubs that stabilize DNA binding and activate transcription.

The paper shows that many transcription factors remain bound to mitotic chromosomes, challenging previous findings of their exclusion and revealing dynamic interactions that depend on DNA binding and nuclear import.