James A. Martin

The paper introduces an automated workflow for high-throughput assembly of large DNA libraries using yeast-based methods, achieving high success rates in assembling diverse DNA constructs.

This paper demonstrates the successful restoration of valine biosynthesis in mammalian cells by integrating synthetic pathways from E. coli, showing that ancient metabolic pathways can be reactivated in metazoans.

The paper transplants the human adenine de novo pathway into yeast to study regulatory mechanisms, finding that PPAT/ADE4 is a central regulatory node influenced by metabolites and mutations.

The paper describes a pipeline for de novo assembly, delivery, and expression of a 101 kb human gene in mouse cells, demonstrating functional evaluation of a full-length gene.