This study relocates a gene cluster to the central chromosomal compartment in Streptomyces, enhancing its transcription and antibiotic production without altering chromosome compaction.

bioRxiv · Mar 4, 2026 · PreprintMicrobiology

A new-engineered integrative tool to target the terminal compartment of the Streptomyces chromosome

Nicolas Delhaye, François R. Pelé, Hoda Jaffal, Sylvie Lautru, Hervé Leh, Stéphanie Bury‐Moné

This paper introduces a new integrative tool, Samy, for targeting the terminal compartment of Streptomyces chromosomes, expanding genetic engineering capabilities.

Applied Microbiology and Biotechnology · Feb 21, 2026Peer-reviewedBioengineering

Identification of the negamycin split biosynthetic gene cluster in Kitasatospora purpeofusca ATCC21470

Alena Strüder, Saad Touzi, Mayarasmi Long, Erik Mingyar, Carolin Schwitalla, Anne Berscheid, Sophie Bourcier, Heike Brötz‐Oesterhelt, Emmanuelle Darbon, Paul Frankenreiter, Mirita Franz‐Wachtel, Christian Geibel, Theresa Anisja Harbig, Chambers C. Hughes, Boris Maček, , Evi Stegmann

This study identifies and characterizes the negamycin biosynthetic gene cluster in Kitasatospora purpeofusca, enabling scalable production through heterologous expression.

bioRxiv · Jul 31, 2025 · PreprintMicrobiology

SYNTERUPTOR: mining genomic islands for non-classical specialised metabolite gene clusters

Drago Haas, Matthieu Barba, Cláudia M. Vicente, Šarká Nezbedová, Amélie Garénaux, Stéphanie Bury‐Moné, Jean-Noël Lorenzi, Laurence Hôtel, Luisa Laureti, Annabelle Thibessard, Géraldine Le Goff, Jamal Ouazzani, Pierre Leblond, Bertrand Aigle, Jean‐Luc Pernodet, , Sylvie Lautru

SYNTERUPTOR identifies genomic islands containing specialised metabolite gene clusters in microbial genomes, detecting clusters missed by existing tools.

NAR Genomics and Bioinformatics · Apr 4, 2024Peer-reviewedBioinformatics