Benedikt Rauscher

The paper maps variant, enhancer, and gene function in primary human CD4+ T cells by combining Perturb-seq of regulatory elements and genes, identifying CRE-gene pairs and linking variants to disease-related programs.

The paper uses single molecule long-read sequencing to analyze transcriptomes in heart failure patients and controls, identifying novel isoforms and convergent isoform changes across different cardiomyopathy types.

This study uses image-based profiling and multi-omics integration to identify morphological axes of variation in colorectal cancer organoids and link them to biological mechanisms and drug responses.

The paper characterizes gene and protein expression changes in NGLY1 deficiency using patient-derived cells and provides a web resource for the data.

This study designs an empirically optimized sgRNA library and experimental protocol to enhance the sensitivity and dynamic range of CRISPR screens for gene essentiality.

The paper introduces a large-scale CRISPR mutagenesis library in Drosophila for targeted gene disruption, showing high efficiency and revealing new developmentally essential genes.

The paper identifies lineage-specific core-regulatory circuits in cancer cells and shows their role in gene essentiality and therapeutic vulnerability.

The paper develops MINGLE, a computational framework to integrate CRISPR/Cas9 screens and identify genetic interactions in cancer cells, revealing new vulnerabilities and building a large genetic network.

CRISPRAnalyzeR is a web application that unifies eight hit-calling methods and 26 external annotation resources to analyze, visualize, and document pooled CRISPR/Cas9 screens without requiring bioinformatics expertise.