Thomas E. Wilson

A CDK1 phospho-switch reprograms TRAIP to unload replisomes in mitosis

The paper identifies a phospho-switch mechanism that reprograms TRAIP to ubiquitylate replisomes in mitosis, enabling disassembly of unreplicated DNA.

High-fidelity rare structural variant detection with HiFiRE3 reduced representation via restriction enzyme ends

HiFiRe3 improves rare structural variant detection by reducing artifacts through error suppression and correction strategies across long and short-read sequencing platforms.

MRE11 suppresses germline mutagenesis at meiotic double-strand breaks in mice

The paper investigates how MRE11 suppresses germline mutagenesis at meiotic double-strand breaks in mice by analyzing the effects of DSB processing and end joining.

Seq-Scope Protocol: Repurposing Illumina Sequencing Flow Cells for High-Resolution Spatial Transcriptomics

This paper describes a protocol to repurpose Illumina sequencing flow cells for high-resolution spatial transcriptomics, enabling detailed spatial gene expression analysis with improved resolution and cost efficiency.

Isoform and pathway-specific post-transcriptional RNA processing in human cells

The paper profiles RNA dynamics in 16 human cell lines using Bru-seq and BruChase-seq, showing gene and pathway-specific RNA turnover and isoform regulation.

POLQ mediates replication-stress induced structural variant formation throughout common fragile sites during mitosis

The paper investigates SV formation at common fragile sites during mitosis and identifies POLQ-mediated end-joining as a key mechanism.

Characterizing nascent transcription patterns of PROMPTs, eRNAs, and readthrough transcripts in the ENCODE4 deeply profiled cell lines

The paper identifies nascent transcription patterns of PROMPTs, eRNAs, and readthrough transcripts in 16 human cell lines using Bru-seq and finds they are regulated by sequence and chromatin features, suggesting they are functionally important.

svCapture: Efficient and specific detection of very low frequency structural variant junctions by error-minimized capture sequencing

The paper introduces svCapture, a method for detecting rare structural variant junctions with high accuracy using error-minimized capture sequencing.

Mapping yeast mitotic 5' resection at base resolution reveals the sequence and positional dependence of nucleases in vivo

Multiple metabolic signals including AMPK and PKA regulate glucose-stimulated double strand break resection in yeast

The paper shows that metabolic signals like AMPK and PKA regulate glucose-stimulated DSB resection in yeast, with multiple pathways contributing to resection efficiency and checkpoint responses.