Joël P.C. Lemiere

Nuclear size and physical properties of the nucleoplasm are determined by colloid osmotic pressure at the nuclear envelope

Overexpressing mCherry-GST targeted to the nucleus or cytoplasm in fission yeast shifts the nuclear-to-cell volume ratio as a parameter-free colloid osmotic model predicts, retuning nucleoplasm diffusivity, condensates and heterochromatin.

Analyses of bent spindles reveal the mechanics of anaphase B in fission yeast

The paper investigates the mechanical properties of fission yeast spindles by analyzing their response to mechanical and genetic perturbations, revealing their behavior as a rigid beam and identifying key proteins involved in elongation and structural integrity.

Phosphorylation of HP1/Swi6 relieves competition with Suv39/Clr4 on nucleosomes and enables H3K9 trimethyl spreading.

This study shows that phosphorylation of HP1/Swi6 reduces competition with Suv39/Clr4 by altering its nucleosome affinity and oligomerization, promoting H3K9me3 spreading in S. pombe.

Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells

The paper compares monomeric StayGold variants using protein nanocages in living cells, finding mStayGold is 3-fold brighter and more photostable than EGFP and other variants.