Derek L. Bolhuis

High-throughput protein target mapping enables accelerated bioactivity discovery for ToxCast and PFAS compounds

The paper uses high-throughput affinity-selection mass spectrometry to identify protein targets of ToxCast and PFAS compounds, discovering novel interactions and revealing distinct binding patterns of legacy and replacement PFAS.

Design of linked-domain protein inhibitors of UBE2D as tools to study cellular ubiquitination

This paper designs a protein inhibitor for UBE2D enzymes to study their role in ubiquitination, showing it reduces Ub degradation and proteotoxic stress in HeLa cells.

Mechanisms of USP18 deISGylation revealed by comparative analysis with its human paralog USP41

This study identifies molecular determinants of USP18's specificity for ISG15 through comparative analysis with its paralog USP41, revealing structural features essential for enzymatic activity.

USP37 prevents unscheduled replisome unloading through MCM complex deubiquitination

This study identifies USP37 as a deubiquitinase that prevents premature replisome disassembly by deubiquitinating MCM7, ensuring genome stability and normal cell cycle progression.