The paper studies the kinetic behavior of the GAGA transcription factor in live Drosophila cells, showing it stably binds chromatin for over 2 minutes, independent of chromatin remodelers.

Nature Structural & Molecular Biology · Jul 1, 2022Peer-reviewedMolecular Biology

Spatio-Temporal Coordination Of Transcription Preinitiation Complex Assembly In Live Cells

Vu Q. C. Nguyen, Anand Ranjan, Sheng Liu, Xiaona Tang, Yick Hin Ling, Jan Wiśniewski, Gaku Mizuguchi, Kai Yu Li, Vivian Jou, Qinsi Zheng, Luke D. Lavis, Timothée Lionnet, Carl Wu

This study uses live-cell imaging in yeast to show how PIC components explore the nucleus and assemble at promoters, revealing spatio-temporal coordination in transcription initiation.

Molecular Cell · Aug 9, 2021Peer-reviewedMolecular Biology

Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction

Anand Ranjan, Vu Q. Nguyen, Sheng Liu, Jan Wiśniewski, Jee Min Kim, Xiaona Tang, Gaku Mizuguchi, Ejlal Elalaoui, Timothy J. Nickels, Vivian Jou, Brian P. English, Qinsi Zheng, Ed Luk, Luke D. Lavis, Timothée Lionnet, Carl Wu

The paper uses live-cell single particle imaging to show that RNA polymerase II and Kin28/Cdk7 kinase are required for H2A.Z eviction in yeast, linking it to transcription processes.

eLife · Apr 27, 2020Peer-reviewedMolecular Biology