This study shows that pigs lacking NKT cells have altered immune responses to influenza, with increased T cell expansion and B cell clone numbers after infection.

PLoS Pathogens · Apr 6, 2026Peer-reviewedImmunology

Compartmentalized cytokine networks and systemic immune remodeling in bovine mammary H5N1 infection.

Gagandeep Singh, Konner R. Cool, Shristi Ghimire, Jessie D. Trujillo, Igor Morozov, Natasha N. Gaudreault, Huibin Lv, Kaijun Jiang, Y. Li, Bumduuren Tuvshintulga, Chester D. McDowell, Adolfo García‐Sastre, Nicholas C. Wu, Jüergen A. Richt

This study investigates immune responses in bovine mammary glands infected with H5N1, finding compartment-specific cytokine networks and systemic immune changes.

bioRxiv · Mar 6, 2026 · PreprintMicrobiology

Temporal dynamics of cytokine, leukocyte, and whole blood transcriptome profiles of pigs infected with African swine fever virus

Daniel W. Madden, Bianca L. Artiaga, Jessie D. Trujillo, Patrícia Akemi Assato, Chester D. McDowell, Isaac Fitz, Taeyong Kwon, Konner R. Cool, Yonghai Li, Natasha N. Gaudreault, Igor Morozov, Jüergen A. Richt

This study tracks cytokine, leukocyte, and transcriptome changes in pigs infected with ASFV, revealing immune dynamics and cellular shifts during disease progression.

Pathogens · Oct 1, 2025Peer-reviewedMicrobiology

Targeted Whole Genome Sequencing of African Swine Fever Virus and Classical Swine Fever Virus on the MinION Portable Sequencing Platform

Chester D. McDowell, Taeyong Kwon, Patrícia Akemi Assato, Emily Mantlo, Jessie D. Trujillo, Natasha N. Gaudreault, Leonardo Cardia Caserta, Igor Morozov, Jayme A. Souza‐Neto, Roman M. Pogranichniy, Diego G. Diel, Jüergen A. Richt

This study develops targeted whole genome sequencing protocols for ASFV and CSFV using MinION sequencing to enable rapid and complete genetic characterization of outbreak strains.

Pathogens · Aug 13, 2025Peer-reviewedMolecular Biology

Pathogenicity and transmissibility of bovine-derived HPAI H5N1 B3.13 virus in pigs

Taeyong Kwon, Jessie D. Trujillo, Mariano Carossino, Heather M. Machkovech, Konner R. Cool, Eu‐Lim Lyoo, Gagandeep Singh, Sujan Kafle, Shanmugasundaram Elango, Govindsamy Vediyappan, Wanting Wei, Nicholas R. Minor, Franco S. Matias-Ferreyra, Igor Morozov, Natasha N. Gaudreault, , Jüergen A. Richt

This study investigates the pathogenicity and transmissibility of a bovine-derived HPAI H5N1 B3.13 virus in pigs, finding susceptibility but limited replication and no transmission.

Emerging Microbes & Infections · May 21, 2025Peer-reviewedMicrobiology

Outcome of H5N1 clade 2.3.4.4b virus infection in calves and lactating cows

Nico Joël Halwe, Konner R. Cool, Angele Breithaupt, Jacob Schön, Jessie D. Trujillo, Mohammed Nooruzzaman, Taeyong Kwon, Ann Kathrin Ahrens, Tobias Britzke, Chester D. McDowell, Ronja Piesche, Gagandeep Singh, Vinícius Pinho dos Reis, Sujan Kafle, Anne Pohlmann, , Jüergen A. Richt

This study investigates H5N1 clade 2.3.4.4b virus infection in calves and lactating cows, finding that mammary gland infection causes severe mastitis and reduced milk production, with transmission likely via milk rather than respiratory routes.

Nature · Sep 25, 2024Peer-reviewedMicrobiology

Bovine highly pathogenic avian influenza virus stability and inactivation in the milk byproduct lactose

Taeyong Kwon, Jordan T Gebhardt, Eu‐Lim Lyoo, Mohammed Nooruzzaman, Natasha N. Gaudreault, Igor Morozov, Diego G. Diel, Jüergen A. Richt

This study shows that H5N1 avian influenza virus remains stable in lactose for 14 days under refrigeration but is inactivated by heat or citric acid.

Viruses · Sep 12, 2024Peer-reviewedMicrobiology