Nicholas C. Wu

Mutational fitness landscapes of diverse human influenza H1N1 neuraminidases

This study quantifies the replication fitness effects of mutations in diverse H1N1 influenza neuraminidases, revealing strain-dependent effects and epistasis patterns.

Antibodies targeting a conserved cryptic epitope at the influenza hemagglutinin head-stem interface via distinct binding modes

This study characterizes two broadly reactive antibodies against a conserved cryptic epitope at the influenza HA head-stem interface, showing they are non-neutralizing and have limited protective efficacy.

Single-cell heterogeneity in interferon induction potential is heritable and governed by variation in cell state

The paper shows that single-cell heterogeneity in IFN induction potential is heritable and driven by baseline variation in JNK and AP-1 signaling states.

Somatic evolution of a cross-reactive germline antibody that expands its breadth to neutralize new SARS-CoV-2 variants

This study identifies a cross-reactive germline antibody that can be evolved to neutralize multiple SARS-CoV-2 variants through targeted somatic mutations.

TRIM21 is a molecular rheostat for influenza A virus replication

This study shows TRIM21 acts as a molecular rheostat regulating influenza A virus replication by modulating ubiquitination and innate immune signaling, with PRKDC as a compensatory antiviral factor when TRIM21 is absent.

Structural insights into antibody responses against influenza A virus in its natural reservoir

This study characterizes antibody responses to H3 influenza A virus in mallard ducks, revealing unique features compared to human antibodies and highlighting gene conversion in antibody evolution.

Compartmentalized cytokine networks and systemic immune remodeling in bovine mammary H5N1 infection.

This study investigates immune responses in bovine mammary glands infected with H5N1, finding compartment-specific cytokine networks and systemic immune changes.

Probing the Role of Membrane in Neutralizing Activity of Antibodies Against Influenza Virus

This study shows that antibodies against influenza virus neutralize by interacting with the viral membrane, and disrupting these interactions reduces their effectiveness.

Characterization of two non-competing antibodies to influenza H3N2 hemagglutinin stem reveals its evolving antigenicity

This study characterizes two non-competing group 2 influenza HA stem antibodies and shows their in vivo protective efficacy despite limited in vitro neutralization.

Structure basis for distinct protective mechanisms of IGHV3-23 antibodies targeting influenza hemagglutinin stem

This study identifies three IGHV3-23 antibodies targeting influenza HA stem, showing that non-neutralizing antibodies with enhanced Fc-mediated effector functions offer better in vivo protection than a potent neutralizer.

Vulnerability in the breadth evolution of an influenza broadly neutralizing antibody

This study investigates the constraints on the breadth evolution of the CR9114 broadly neutralizing antibody by comparing binding affinity landscapes across different influenza HA subtypes.

Evolution of antibody cross-reactivity to influenza H5N1 neuraminidase from an N2-specific germline

This study identifies an antibody that cross-reacts with H5N1 and H3N2 neuraminidases and shows how somatic hypermutation enables this cross-reactivity.

High-throughput synthesis and specificity characterization of natively paired antibodies using oPool+ display

This study introduces oPool+ display, a high-throughput platform for synthesizing and characterizing natively paired antibodies, demonstrating its application in analyzing influenza HA antibodies.

Mapping antigenic evolution of influenza A virus using deep learning-based prediction of hemagglutination inhibition titers

This paper uses a deep learning model to predict hemagglutination inhibition titers from influenza virus sequences, enabling large-scale antigenic mapping and identifying evolutionary patterns in A(H1N1)pdm and A(H3N2) viruses.

Intrinsic OASL expression licenses interferon induction during influenza A virus infection

The paper identifies intrinsic OASL expression as a determinant of IFN induction potential in influenza A virus-infected cells using scRNA-seq analysis.

Systematic Investigation of Double Emulsion Dewetting Dynamics for the Droplet Microfluidic Production of Giant Unilamellar Vesicles (GUVs) under Biocompatible Conditions

This study investigates the factors affecting GUV production via double emulsion microfluidics and shows that high glycerol and P188 concentrations are unnecessary, enabling more reliable and accessible GUV production.

Deep mutational scanning of influenza A virus NEP reveals pleiotropic mutations in its N-terminal domain

This study uses deep mutational scanning to analyze the functional impact of NEP mutations in influenza A virus, revealing pleiotropic effects on viral replication and host adaptation.

Seasonal influenza A virus lineages exhibit divergent abilities to antagonize interferon induction and signaling

This study compares the ability of seasonal H1N1 and H3N2 influenza A viruses to antagonize interferon induction and signaling at the single-cell level, finding differences in their IFN antagonism potential.

Differential antigenic imprinting effects between influenza H1N1 hemagglutinin and neuraminidase in a mouse model

This study examines how immune history affects antibody responses to influenza H1N1 hemagglutinin and neuraminidase in mice, finding that HA responses are more restricted by prior infection than NA responses.

A library-on-library screen reveals the breadth expansion landscape of a broadly neutralizing betacoronavirus antibody

This study uses a library-on-library approach to analyze how a betacoronavirus antibody evolves breadth through somatic hypermutations, identifying key mutations that improve binding across multiple antigenic variants.

Natural variation in neuraminidase activity influences the evolutionary potential of the seasonal H1N1 lineage hemagglutinin

This study shows that natural variation in neuraminidase activity affects the evolutionary potential of hemagglutinin in seasonal H1N1 influenza viruses.

Combinatorial in silico approach for cancer-associated 4Fe-4S protein discovery

This study identifies potential cancer-associated 4Fe-4S proteins using three computational traits, discovering 21 novel candidates beyond known ones.

Stringent and complex sequence constraints of an IGHV1-69 broadly neutralizing antibody to influenza HA stem

This study identifies sequence constraints for IGHV1-69 antibodies targeting influenza HA stem, showing light chain and CDR H3 sequences coordinate HA stem specificity.

Benchmarking of computational error-correction methods for next-generation sequencing data

This study benchmarks error-correction methods for next-generation sequencing data, finding significant variation in performance across datasets with no single method being optimal overall.

MiCoP: Microbial Community Profiling method for detecting viral and fungal organisms in metagenomic samples

MiCoP improves microbial community profiling by accurately detecting viral and fungal organisms in metagenomic samples using a comprehensive reference database.

Bacterial Glycosyltransferase-mediated Cell-surface Chemoenzymatic Glycan Editing: Methods and Applications

This paper introduces bacterial glycosyltransferases for cell-surface glycan editing and applies them to study influenza A virus infection mechanisms.

Adaptive potential of a drug-targeted viral protein as a function of environmental stress

The paper quantifies the adaptive potential of HCV NS5A protein mutations under drug selection and environmental stress, finding increased beneficial mutations and fitness costs.

Adaptation in protein fitness landscapes is facilitated by indirect paths

The paper experimentally characterizes a protein fitness landscape, finding that indirect adaptation paths through extra dimensions can bypass evolutionary traps caused by epistasis.

High-throughput functional annotation of influenza A virus genome at single-nucleotide resolution

This study presents a platform for high-throughput functional annotation of influenza A virus genome mutations, identifying critical residues and validating mutation effects with high accuracy.