Diego G. Diel

Infection and transmission dynamics of bovine and human influenza A H5N1 viruses in mouse and hamster models

This study compares the pathogenicity and transmissibility of bovine- and human-derived H5N1 viruses in mouse and hamster models, finding high pathogenicity but limited transmissibility.

Pathogenesis of H5N1 Clade 2.3.4.4b in dry Jersey cows following intramammary inoculation shows within-host compartmentalization

This study shows that dry Jersey cows are highly susceptible to H5N1 HPAIV, with virus dose affecting mastitis severity in udder quarters.

Characterization of bovine-derived H5N1 viruses expressing fluorescent and luminescent reporter proteins

This study creates H5N1 reporter viruses with fluorescent and luminescent markers to study viral replication and antiviral responses in cattle and mice.

The ORF6 accessory protein contributes to SARS-CoV-2 virulence and pathogenicity in the naturally susceptible feline model of infection

This study shows that deleting ORF6 from SARS-CoV-2 reduces virulence and pathogenicity in cats, with decreased immune suppression and reduced viral replication.

Hematogenous neuroinvasion and genotype-dependent transmission of influenza A H5N1 viruses in the cat host

This study examines H5N1 influenza virus pathogenesis and transmission in cats, showing genotype-dependent differences in disease severity, shedding, and transmissibility.

Unexpected Detection of Highly Pathogenic Avian Influenza (HPAI) H5N1 virus in bovine semen from a bull used for natural breeding on an affected dairy farm

The paper reports the detection of HPAI H5N1 virus RNA in bovine semen from an asymptomatic bull during an outbreak, raising concerns about transmission risks and biosecurity.

Targeted Whole Genome Sequencing of African Swine Fever Virus and Classical Swine Fever Virus on the MinION Portable Sequencing Platform

This study develops targeted whole genome sequencing protocols for ASFV and CSFV using MinION sequencing to enable rapid and complete genetic characterization of outbreak strains.

Digital Immunoassay for Rapid Detection of SARS-CoV-2 Infection in a Broad Spectrum of Animals

This paper develops a digital immunoassay for rapid detection of SARS-CoV-2 antibodies in multiple animal species with high sensitivity and specificity.

Pathogenicity and transmissibility of bovine-derived HPAI H5N1 B3.13 virus in pigs

This study investigates the pathogenicity and transmissibility of a bovine-derived HPAI H5N1 B3.13 virus in pigs, finding susceptibility but limited replication and no transmission.

Emergence and interstate spread of highly pathogenic avian influenza A(H5N1) in dairy cattle

This study identifies a reassortment event in wild birds leading to HPAI A(H5N1) infection in cattle and highlights the risk of virus spread through asymptomatic cattle to humans.

Thermal inactivation spectrum of influenza A H5N1 virus in raw milk

This study evaluates the thermal inactivation of HPAI H5N1 virus in raw milk under various conditions, finding efficient inactivation except for certain thermization protocols.

A multiplex real-time PCR assay for detection of equine herpesvirus 1 and equine herpesvirus 4

This paper develops and validates a multiplex real-time PCR assay for simultaneous detection of EHV-1 and EHV-4 in horses, showing improved specificity and 100% concordance with singleplex assays.

Stability of influenza A H5N1 virus in raw milk cheese

This study evaluates the stability of H5N1 virus in raw milk cheese under different pH conditions and finds that the virus persists for up to 60 days at higher pH levels but not at lower pH.

Naturally occurring N-terminal mutations in SARS-CoV-2 nsp1 impact innate immune modulation but do not affect virus virulence

This study identifies two N-terminal deletions in SARS-CoV-2 nsp1 that reduce innate immune evasion but do not impact viral virulence.

Outcome of H5N1 clade 2.3.4.4b virus infection in calves and lactating cows

This study investigates H5N1 clade 2.3.4.4b virus infection in calves and lactating cows, finding that mammary gland infection causes severe mastitis and reduced milk production, with transmission likely via milk rather than respiratory routes.

Bovine highly pathogenic avian influenza virus stability and inactivation in the milk byproduct lactose

This study shows that H5N1 avian influenza virus remains stable in lactose for 14 days under refrigeration but is inactivated by heat or citric acid.

From birds to mammals: spillover of highly pathogenic avian influenza H5N1 virus to dairy cattle led to efficient intra- and interspecies transmission

This study reports the spillover of HPAI H5N1 virus to dairy cattle, showing efficient intra- and interspecies transmission and viral tropism in mammary gland epithelial cells.

Rapid genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) using MinION nanopore sequencing

This paper develops and optimizes MinION nanopore sequencing methods for rapid genotyping of PRRSV, achieving high sequence identity and coverage in clinical samples.

The United States Swine Pathogen Database: integrating veterinary diagnostic laboratory sequence data to monitor emerging pathogens of swine

The paper develops a centralized database for swine pathogen sequences to monitor emerging pathogens and support genomic surveillance.