Adolfo García‐Sastre

Coevolutionary constraints of Zika virus nonstructural protein 5 replication and interferon antagonism activities

This study identifies evolutionary constraints linking Zika virus NS5 replication and interferon antagonism by analyzing fitness landscapes and attenuation in mice.

MDL-001: an Oral, Direct-Acting Broad-Spectrum Antiviral for Influenza-Like Illness (ILI) and Chronic Hepatitis In Vivo

This paper evaluates MDL-001, a broad-spectrum antiviral, showing potent in vitro and in vivo activity against influenza, RSV, SARS-CoV-2, and hepatitis B/C/D viruses, with improved efficacy compared to existing treatments.

Evaluation of new adjuvants in enhancing the immune response to a candidate of Severe Fever with Thrombocytopenia Syndrome Virus vaccine.

This study evaluates adjuvants for a SFTSV vaccine, finding that Addavax and SDI enhance neutralizing antibodies and immune memory, with NP as a key target.

Small molecule inhibition of the mitochondrial lipid transfer protein STARD7 attenuates influenza viral replication

This paper identifies a small molecule, M4, that inhibits influenza A and B replication by targeting the host protein STARD7, preventing vRNP nuclear export and demonstrating synergistic antiviral effects with baloxavir in mice.

A bireporter recombinant SARS-CoV-2 Omicron BA.5 for in vitro and in vivo studies

This paper develops a bireporter recombinant SARS-CoV-2 Omicron BA.5 strain for tracking viral infections and testing antivirals and neutralizing antibodies in vitro and in vivo.

IMPAIRED NEURAMINIDASE AND POLYMERASE ACTIVITIES CORRESPOND WITH LIMITED AEROSOL INFECTIVITY OF B3.13 AND D1.1 H5N1 LINEAGES IN HUMAN RESPIRATORY CULTURES

This study shows that H5N1 viruses from B3.13 and D1.1 lineages have reduced infectivity in human respiratory cultures due to impaired neuraminidase and polymerase activities, suggesting lower pandemic risk than traditional models indicate.

Mutations in SARS-CoV-2 Nsp1 are critical determinants of viral pathogenicity in mice

This study identifies critical residues in SARS-CoV-2 Nsp1 that determine viral pathogenicity in mice by reducing replication and immune response severity.

Enhanced Pathogenicity and Contact Transmissibility of Human-origin Avian Influenza H5N1 Clade 2.3.4.4b Genotype B3.13 Compared to D1.1 in Ferrets

This study compares the pathogenicity and transmissibility of two H5N1 genotypes in ferrets, finding B3.13 is more pathogenic and transmissible than D1.1, while D1.1 acquires adaptive mutations rapidly.

A One-Shot Multivalent Live-Attenuated Candidate Influenza Vaccine against Divergent Zoonotic H5N1 Clades

This paper develops a multivalent live-attenuated influenza vaccine that provides broad protection against multiple H5N1 clades by incorporating HA and NA from representative clades, showing robust immune responses and protection in mice.

Identification and characterization of a SARS-CoV-2 Mpro G23 deletion ensitrelvir-resistant mutant

This study identifies and characterizes a SARS-CoV-2 Mpro G23 deletion mutant resistant to ensitrelvir but not to nirmatrelvir or remdesivir.

XIAP-associated factor 1 protects against viral-induced neuropathogenesis.

This study shows XAF1 protects against viral-induced neuropathogenesis by limiting excessive IFN responses in neurotropic virus infections.

Zoonotic infections and genomic evolution associated with novel reassortants swine-origin influenza A viruses in Spain

This study analyzes the genomic evolution and zoonotic potential of swine-origin influenza A viruses in Spain, identifying novel reassortants and highlighting human-swine viral transmission.

Coronavirus protein interaction mapping in bat and human cells identifies molecular and genetic switches for immune evasion and replication

Entry, replication and innate immunity evasion of BANAL-236, a SARS-CoV-2-related bat virus, in Rhinolophus and human cells

This study investigates BANAL-236, a SARS-CoV-2-related bat virus, showing it can use human entry factors and evade human innate immunity, suggesting potential for zoonotic spillover.

Compartmentalized cytokine networks and systemic immune remodeling in bovine mammary H5N1 infection.

This study investigates immune responses in bovine mammary glands infected with H5N1, finding compartment-specific cytokine networks and systemic immune changes.

Innate antiviral readiness drives the expansion of protective T stem cell memory against influenza

This study identifies innate antiviral readiness as a key driver of TSCM cell expansion, showing its predictive value for viral clearance and vaccine design.

One Thousand SARS-CoV-2 Antibody Structures Reveal Convergent Binding and Near-Universal Immune Escape

The paper analyzes 1,100 SARS-CoV-2 antibody structures to show convergent paratope structures and near-universal immune escape by advanced variants.

A cocktail of SARS-CoV-2 spike stem helix domain and receptor binding domain human monoclonal antibodies prevent the emerge of viral escape mutants

This study shows that a combination of antibodies targeting both the SARS-CoV-2 spike protein's receptor binding domain (RBD) and stem helix (SH) domains prevents the emergence of viral escape mutants.

Bioluminescent reporter influenza A viruses to track viral infections

This paper develops recombinant influenza A viruses expressing nanoluciferase for in vitro and in vivo tracking of viral infections, showing they replicate efficiently and can be imaged using IVIS.

ISG15 Differentially Modulates Clade Ib and II MPXV Infection in MEF cells

This study shows ISG15 modulates Clade Ib and II MPXV infection differently in MEF cells, with clade-specific effects on viral replication and immune evasion.

Lymphangiogenesis driven by VEGF-C reshapes tumor immune landscape and enables tumor eradication by viral immunotherapy

This study shows that VEGF-C promotes lymphangiogenesis in tumors, enhancing immune cell infiltration and enabling complete tumor eradication with viral immunotherapy.

Single-Cell Network Analysis Identifies CLEC4E as a Key Mediator of Proinflammatory mDC Responses in Influenza Infection

This study identifies CLEC4E as a key driver of pro-inflammatory mDC responses in influenza using single-cell network analysis and validates its therapeutic potential in a murine model.

A Human H5N1 Influenza Virus Expressing Bioluminescence for Evaluating Viral Infection and Identifying Therapeutic Interventions

This paper develops a bioluminescent H5N1 influenza virus to track infection and test antiviral drugs in vitro and in vivo.

Dose-dependent interferon programs in myeloid cells after mRNA and adenovirus COVID-19 vaccination

This study compares immune responses to different COVID-19 vaccines in humans, finding distinct interferon programs in myeloid cells based on vaccine type and dose timing.

Broad-spectrum RNA antiviral inspired by ISG15-/- deficiency

The paper identifies a set of 10 ISGs that confer broad-spectrum antiviral resistance and demonstrates their efficacy in protecting against multiple viruses in vivo.

Differential modulation of Interferon and Cell Death Responses defines Human vs Avian Influenza A Virus Strain-Specific Virulence and guides Combination Therapy.

This study identifies strain-specific virulence mechanisms in IAV and proposes a combination therapy using Oseltamivir and Baricitinib to reduce inflammation and improve survival in a murine model.

CMV-specific clonal expansion of Th1, GZMK⁺ CD8⁺, and TEMRA T cells revealed by human PBMC single cell profiling

The paper uses single-cell profiling to show CMV drives clonal expansion of GZMK CD8 and Th1 cells in humans.

Addressing SARS-CoV-2 Evolution: Neutralization of Emerging Variants of Concern by the AVX/COVID-12 'Patria' Vaccine Based on HexaPro-S Ancestral Wuhan Spike or Its Updated BA.2.75.2 Version

This study evaluates two SARS-CoV-2 vaccines, AVX/COVID-12 (V-Wu) and V-BA, showing V-BA provides better protection against Omicron subvariants while V-Wu offers broader coverage against multiple variants.

Identification and Targeting of Regulators of SARS-CoV-2-Host Interactions in the Airway Epithelium.

The paper identifies master regulator modules in human airway epithelial cells reprogrammed by SARS-CoV-2 and finds 11 drugs that reverse these signatures.

Deletion of a KSF Motif Attenuates NSP1 Host Cell Translation Shutoff and Impairs SARS-CoV-2 Virulence

This study shows that a deletion in the NSP1 protein of SARS-CoV-2 variants reduces virulence by impairing host translation shutoff, suggesting NSP1 as a target for antiviral strategies.