Troy C. Krzysiak

An efficient system for production of highly purified inhibins using mammalian cell expression and tandem affinity purification

This paper develops a method to produce highly purified inhibins using mammalian cell expression and tandem affinity purification, achieving high yields and purity with minimal activin contamination.

Pro-domain-dependent folding and co-receptor-mediated targeting to optimize an antagonistic TGF-β monomer for gene-based delivery

This paper improves the folding and targeting of an engineered TGF-β monomer for gene-based delivery by modifying its pro-domain and adding a CD44 binding domain.

Structures of TGF-beta with betaglycan and the signaling receptors reveal the mechanism whereby betaglycan potentiates receptor complex assembly and signaling

This study determines the structure of TGF-beta bound to betaglycan and its signaling receptors, revealing mechanisms of ligand selectivity and signal potentiation.