The paper identifies a noncanonical miRNA biogenesis pathway in C. elegans and humans that is Pol III-dependent, Microprocessor-independent, and Dicer-dependent, generating germline-enriched miRNAs like mir-1829 and miR-4521.

4 citations

C. eleganshuman

𝕏 PostBluesky

Molecular BiologyNucleic Acids ResearchPeer-reviewedFeb 27, 2024

The catalytic activity of microRNA Argonautes plays a modest role in microRNA star strand destabilization in C. elegans

Kasuen Kotagama, Acadia L. Grimme, Leah Braviner…·Katherine McJunkin

This study shows that miRNA Argonaute slicing has a minor role in star strand destabilization in C. elegans, with limited developmental impact and context-dependent effects.

7 citations

C. elegansexperimental assay

𝕏 PostBluesky

Molecular BiologybioRxivPreprintFeb 5, 2024

A POLR3B-variant reveals a Pol III transcriptome response dependent on La protein/SSB

Sandy Mattijssen, Kyra Kerkhofs, Joshi Stephen…·Richard J Maraia

The paper identifies a POLR3B variant causing mis-splicing and altered Pol III transcriptome, revealing La protein's role in reshaping small ncRNA profiles and identifying tRF-1/tRF-3 as biomarkers for POLR3 deficiency.

DOI 10.1101/2024.02.05.577363

humanbulk RNA-seq

𝕏 PostBluesky

GeneticsG3 Genes Genomes GeneticsPeer-reviewedAug 20, 2020

Identification of essential genes and fluconazole resistance genes in Candida glabrata by profiling of Hermes transposon insertions.

Andrew N. Gale, Rima M. Sakhawala, Anton Levitan…·Kyle W. Cunningham

The paper identifies essential genes and fluconazole resistance genes in Candida glabrata using Hermes transposon insertion profiling and machine learning.

56 citations

C. elegansmachine learning (classical)

𝕏 PostBluesky