Ulises H. Guzmán

Orbitrap Astral Zoom upgrades enable 270 Hz DIA scanning, identifying 7184 protein groups at 500 samples/day and >12,000 proteins from fractionated HEK293 in 2.7 h.

This paper introduces a high-throughput spatial proteomics workflow for FFPE samples, enabling efficient processing and analysis of subcellular regions with high resolution.

This paper introduces a new scanning strategy called pre-accumulation to enhance ion utilization and sensitivity in Orbitrap mass spectrometers, improving peptide and protein identification in high-throughput proteomics.

This study introduces SC-pSILAC, a method to measure protein abundance and turnover in single cells, revealing differentiation-specific dynamics and cell-type markers in human iPSCs.

This study improves single-cell proteomics by increasing protein identification from ~2000 to over 5000 per cell and enhancing throughput and reliability.

The paper uses spatiotemporal proteomics to identify how different EGFR ligands induce distinct signaling outcomes, revealing key proteins and phosphorylation sites involved in endocytic fate and functional selectivity.

The paper introduces Narrow-window DIA, a fast proteomics method enabling high-throughput and comprehensive proteome analysis with high precision and accuracy.