Leander van der Hoeven

This study maps the human sperm proteome across compartments, donors, and individual cells, revealing conserved and variable proteins and linking mitochondrial dysfunction to male infertility.

This study reveals the HSP90-CDC37 chaperone system's role in RAF1 activation through pre-dimerization and asymmetric folding, showing its active regulatory role in MEK signaling and cell proliferation.

This study compares three LysC homologues for proteomics, finding A. lyticus LysC provides better peptide identification and digestion efficiency than others.

This study improves single-cell proteomics by increasing protein identification from ~2000 to over 5000 per cell and enhancing throughput and reliability.

The paper introduces hybrid-DIA, a method combining targeted and discovery phosphoproteomics for single spheroids, showing comparable accuracy to existing methods while profiling the global phosphoproteome.