Daniel J. Hermanson

Higher-Throughput Proteome Profiling Enabled by Parallelized Pre-Accumulation and Optimized Ion Processing in the Orbitrap Astral Zoom Mass Spectrometer

Orbitrap Astral Zoom upgrades enable 270 Hz DIA scanning, identifying 7184 protein groups at 500 samples/day and >12,000 proteins from fractionated HEK293 in 2.7 h.

A Single-Aliquot, Enrichment-Free Workflow for High-Throughput Plasma Proteome and N-Glycoproteome Profiling

This paper introduces a single-aliquot, enrichment-free workflow for high-throughput plasma proteome and N-glycoproteome profiling, achieving deep coverage and reproducibility in a clinical setting.

From HeLa to human blood: extending single-cell proteomics to the smallest immune cells

This paper develops a high-sensitivity single-cell proteomics platform and applies it to analyze small immune cells, achieving deep proteome coverage and identifying key markers in PBMCs.

Whole blood proteome dynamics defines predictive diagnostic and prognostic signatures of cryptococcal infection

Spatiotemporal dynamics of cryptococcal infection reveal novel immune modulatory mechanisms and antifungal targets

This study uses mass spectrometry to analyze proteome changes in three organs during Cryptococcus neoformans infection, identifying new immune modulatory mechanisms and antifungal targets.

Ultra-Fast Multi-Organ Proteomics Unveils Tissue-Specific Mechanisms of Drug Efficacy and Toxicity

The paper introduces UFP-MS, a fast proteomics method, and applies it to study drug effects on mouse tissues, revealing tissue-specific metabolic changes.

Narrow-window DIA: Ultra-fast quantitative analysis of comprehensive proteomes with high sequencing depth

The paper introduces Narrow-window DIA, a fast proteomics method enabling high-throughput and comprehensive proteome analysis with high precision and accuracy.