Tetiana Gren

The paper identifies a new PCR artifact called 'PCR leaping' and develops a DNA library screening method using it for natural product discovery.

The paper introduces a type I-C CRISPR system, pCRISPR-Cas3, for efficient genome engineering in streptomycetes, outperforming Cas9-based systems in deletion and substitution capabilities.

The paper reports the discovery of maramycin, a novel isoquinolinequinone alkaloid produced through heterologous expression of a bifunctional enzyme in Streptomyces albidoflavus, showing moderate cytotoxicity against prostate cancer cells.

The paper uses independent component analysis to characterize the transcriptional regulatory network of Streptomyces albidoflavus and identifies iModulons that describe its activity across growth conditions.

The paper develops CRISPR-BEST, a DSB-free base editing system for filamentous actinomycetes, achieving single-nucleotide resolution in Streptomyces species.