Choong Tat Keng

This paper introduces Spatial Perturb-Seq, a CRISPR-based method to screen genes in intact tissues, revealing gene effects on neuronal communication in mouse brains.

This study demonstrates the feasibility of using dual-ssAAV6 vectors for in vivo gene delivery to HSC from thalassemia patients in a humanized mouse model, showing efficient transduction and long-term persistence.

The paper develops a method to measure transduction efficiency and specificity of viral vectors in complex cell populations using single-cell RNA sequencing.