Wei Leong Chew

This paper introduces Spatial Perturb-Seq, a CRISPR-based method to screen genes in intact tissues, revealing gene effects on neuronal communication in mouse brains.

The paper identifies conserved GABAergic neurons in zebrafish and mouse lateral hypothalamus that respond to growth hormone and food cues, revealing a shared mechanism for appetite regulation.

This study demonstrates the feasibility of using dual-ssAAV6 vectors for in vivo gene delivery to HSC from thalassemia patients in a humanized mouse model, showing efficient transduction and long-term persistence.

The paper develops a method to measure transduction efficiency and specificity of viral vectors in complex cell populations using single-cell RNA sequencing.

This paper develops C:G to G:C base editors to correct specific SNPs in human cells, achieving up to 37% editing efficiency and 90% specificity.

The paper explores immune orthogonality among protein orthologs by analyzing CRISPR-Cas9 and AAV serotypes, finding high immune orthogonality in Cas9 but not in AAVs.

This paper introduces targeted deaminases for site-specific C-to-T genomic edits without DNA cleavage, achieving 13% efficiency in E. coli and 2.5% in human cells with reduced toxicity.