Andrej Lupták

A miniature bioluminescent platform for imaging structured RNAs

This paper develops a miniaturized bioluminescent platform for imaging structured RNAs in live cells, achieving a 3-fold reduction in tag size compared to existing methods.

Engineering a ribozyme with aminoacyl-tRNA synthetase activity

This paper engineers a ribozyme that can aminoacylate tRNA and discriminate between cognate and non-cognate tRNAs, enabling non-canonical amino acid incorporation.

An open, integrated platform for multiplexed bioluminescence microscopy

The paper introduces an open platform for multiplexed bioluminescence and fluorescence microscopy, including tools for experiment design, microscope upgrades, and data analysis.

Bioorthogonal cyclopropenones for investigating RNA structure

This paper develops bioorthogonal cyclopropenones to crosslink RNA and study its native structures in cells.

Inhibition of CPEB3 ribozyme elevates CPEB3 protein expression and polyadenylation of its target mRNAs, and enhances object location memory

The paper shows that inhibiting the CPEB3 ribozyme increases CPEB3 protein and enhances memory by promoting mRNA polyadenylation and translation.

A modular platform for bioluminescent RNA tracking

This paper introduces a bioluminescent platform for tracking RNA in vivo, demonstrating single-copy detection and robust imaging in cells and live animals.

The CPEB3 ribozyme modulates hippocampal-dependent memory

The paper shows that the CPEB3 ribozyme regulates hippocampal-dependent memory by modulating mRNA maturation and translation of plasticity-related genes.

Regulation of mRNA translation by a photoriboswitch

The paper develops a light-responsive RNA riboswitch that reversibly controls gene expression by photoisomerizing a small molecule ligand.