Sara P. Garcia

ONE-seq identifies population-specific off-target effects of CRISPR gene editors using global human genome sequence diversity.

The paper benchmarks 10 scATAC-seq analysis methods on synthetic and real datasets, finding SnapATAC, Cusanovich2018, and cisTopic perform best in separating cell populations across varying coverage and noise levels.

The paper describes SECURE-ABE and -CBE base editors with reduced RNA off-target activities and improved DNA editing precision.

The paper introduces VIVO, a method to detect off-target mutations from in vivo CRISPR-Cas editing, showing that well-designed gRNAs can edit efficiently without detectable off-targets.