Jimmy A. Guo

GLIS3 Marks a Neural-like Progenitor Cell State that Drives Metastasis in Pancreatic Ductal Adenocarcinoma

This study identifies Glis3 as a key transcription factor driving metastasis in PDAC by promoting a neural-like progenitor cell state.

Distinct malignant cell states and myeloid glutamate signaling associated with aggressive pancreatic neuroendocrine tumors

This study identifies distinct malignant cell states and myeloid glutamate signaling in aggressive pancreatic neuroendocrine tumors, linking them to poor clinical outcomes and therapeutic vulnerabilities.

The Pdgfd-Pdgfrb axis orchestrates tumor-nerve crosstalk in pancreatic cancer

This study identifies Pdgfd-Pdgfrb signaling as a driver of perineural invasion in pancreatic cancer by promoting cancer cell invasiveness and nerve engagement.

Global-scale CRISPR gene editor specificity profiling by ONE-seq identifies population-specific, variant off-target effects

ONE-seq identifies population-specific off-target effects of CRISPR gene editors using global human genome sequence diversity.

Single-nucleus and spatial transcriptomics of archival pancreatic cancer reveals multi-compartment reprogramming after neoadjuvant treatment

This study uses single-nucleus and spatial transcriptomics to analyze pancreatic cancer before and after neoadjuvant treatment, revealing changes in cell states and immune infiltration that may inform therapy.

CRISPR-SURF: Discovering regulatory elements by deconvolution of CRISPR tiling screen data

CRISPR-SURF deconvolves CRISPR tiling screen data to identify functional regulatory elements across CRISPR-Cas, CRISPRi, and CRISPRa experiments.

In vivo CRISPR-Cas gene editing with no detectable genome-wide off-target mutations

The paper introduces VIVO, a method to detect off-target mutations from in vivo CRISPR-Cas editing, showing that well-designed gRNAs can edit efficiently without detectable off-targets.