Karl Petri

Gene editing without ex vivo culture evades genotoxicity in human hematopoietic stem cells

This study shows that gene editing of HSCs without ex vivo culture reduces genotoxicity while maintaining therapeutic efficacy for beta-hemoglobinopathy treatment.

Enhancing CRISPR prime editing by reducing misfolded pegRNA interactions

This study identifies pegRNA misfolding as a factor limiting CRISPR prime editing efficiency and shows refolding and point mutations can significantly improve it in zebrafish embryos.

Global-scale CRISPR gene editor specificity profiling by ONE-seq identifies population-specific, variant off-target effects

ONE-seq identifies population-specific off-target effects of CRISPR gene editors using global human genome sequence diversity.

PrimeDesign software for rapid and simplified design of prime editing guide RNAs

PrimeDesign is a tool for designing prime editing guide RNAs, enabling single and combination edits, and it supports the creation of a database for over 68,500 pathogenic variants.