David M. Tobin

The paper identifies pks12 as an in vivo essential gene in Mtb by combining genomic, metabolomic, and phenotypic analyses, revealing its role in mycolyl lipid synthesis and host-pathogen interface structure.

This study shows that Mycobacterium marinum switches ESX-1 substrates in response to acidic pH, affecting phagosome lysis and bacterial survival.

The paper uses dual RNA-seq in zebrafish to study transcriptional programs in tuberculous granulomas, finding neutrophils and necrosis drive bacterial survival and adaptation.

The paper maps unknown lipids in bacterial pathogens using lipidomic methods, revealing new biosynthetic products linked to virulence.

This study identifies cathepsin Z as a susceptibility factor for tuberculosis severity in both mice and humans, showing its role in disease outcomes through genetic and cellular analyses.

This study identifies EspN as a critical transcription factor regulating the ESX-1 system in M. marinum, which is essential for macrophage infection and disease burden, but its activity is masked by EspM under laboratory conditions.

This paper develops a CRISPR/Cas9 system for neutrophil-specific gene disruption in zebrafish and demonstrates its utility in studying gene function.