Satyam Tiwari

This paper describes a BSL-2 compatible purification protocol for full-length TDP-43 using refolding-assisted IMAC, enabling safe and reproducible production for in vitro studies.

The paper investigates how J-domain proteins in yeast regulate Hsp70 chaperones through intramolecular interactions, showing auto-regulatory effects that reduce ATPase cycles.

This study uses a fluorescent reporter substrate to elucidate the stepwise mechanism of Hsp70 unfoldase action in converting aggregates into native proteins.

The paper shows that GroEL/ES buffers entropic traps in protein folding pathways introduced by mutations, which are rescued by the chaperonin, while it can also edit non-native contacts but is not utilized in vivo.