Benjamin Furtwängler

Defining Quality Control Standards for Single-Cell Proteomics by Inter-Laboratory Benchmarking

This study establishes quality control standards for single-cell proteomics through inter-laboratory benchmarking, identifying software and technical factors affecting data reliability.

Mapping the human hematopoietic stem and progenitor cell hierarchy through integrated single-cell proteomics and transcriptomics

The paper integrates single-cell proteomics and transcriptomics to study human hematopoietic stem and progenitor cells, identifying protein markers and dynamics not captured by mRNA alone.

Informed Data-Independent Acquisition Enables Targeted Quantification of Key Regulatory Proteins in Cell Fate Decisions at Single-Cell Resolution

The paper introduces iDIA, a method that enhances sensitivity for quantifying regulatory proteins in single cells, revealing lineage priming and cell-cycle coordination in hematopoietic stem and progenitor cells.

Quantitative Label-Free Single-Cell Proteomics on the Orbitrap Astral MS

This study evaluates the Orbitrap Astral MS's sensitivity for single-cell proteomics, showing improved proteome coverage and quantitative accuracy in low-input samples.

Unraveling the proteome landscape of mouse hematopoietic stem and progenitor compartment with high sensitivity low-input proteomics

This study develops a low-input proteomics workflow to analyze rare hematopoietic stem and progenitor cells, identifying 7,000 proteins and a novel marker for long-term hematopoietic stem cells.

Evaluating the capabilities of the Astral mass analyzer for single-cell proteomics

This study evaluates the Astral mass analyzer's performance in single-cell proteomics using DIA, showing improved proteome depth and quantitative precision for ultra-low input samples.

Enhancing single-cell proteomics through tailored Data-Independent Acquisition and micropillar array-based chromatography

The paper develops a tailored DIA method for single-cell proteomics to improve quantification from low-input samples and establishes a complete scp-MS workflow.

High sensitivity limited material proteomics empowered by data-independent acquisition on linear ion traps

The paper develops a method combining linear ion traps and FAIMS for low-input proteomics, improving sensitivity and throughput for limited material samples.

Quantitative Single-Cell Proteomics as a Tool to Characterize Cellular Hierarchies

The paper presents a workflow for single-cell proteomics to study cellular heterogeneity in leukemia, demonstrating the ability to quantify thousands of proteins per cell.

Mapping physiological ADP-ribosylation using Activated Ion Electron Transfer Dissociation (AI-ETD)

The paper introduces AI-ETD for MS-based proteomics analysis of physiological ADP-ribosylation, identifying more ADPr sites than existing methods.