Osvaldo Burastero

This study benchmarks DSF across labs, showing variability in thermal stability parameters due to instruments, workflows, and fluorescence readouts, with harmonization and centralized analysis improving consistency.

The paper introduces CheMelt, a tool for analyzing thermal and chemical denaturation of proteins, and shows de novo designed proteins have lower thermodynamic parameters than natural proteins.

ChiraKit is an online tool that processes and analyzes circular dichroism data to calculate secondary structure, estimate helicity, fit unfolding models, and decompose spectra.

The paper investigates clathrin binding selectivity among yeast adaptor proteins, finding Ent5 has the highest affinity and Ent1 shows stronger clathrin interaction than Ent2.