Magnus Kjærgaard

Global analysis of thermal and chemical denaturation using CheMelt: Thermodynamic dissection of highly thermostable de novo designed proteins

The paper introduces CheMelt, a tool for analyzing thermal and chemical denaturation of proteins, and shows de novo designed proteins have lower thermodynamic parameters than natural proteins.

Affinity and distance dependence of SLiM-mediated dephosphorylation by protein phosphatase 1

The paper quantifies how SLiM affinity and distance affect PP1-mediated dephosphorylation, showing optimal spacing and affinity for catalytic efficiency.

Sequence determinants of the hypomobility of intrinsically disordered proteins in SDS-PAGE

The paper identifies sequence determinants affecting the hypomobility of intrinsically disordered proteins in SDS-PAGE using synthetic low complexity domains.

Oxygen partitioning into biomolecular condensates is governed by protein density

The paper investigates oxygen partitioning into biomolecular condensates and finds that oxygen concentration varies with protein density and chain length.

Slow diffusion limits phosphorylation in a biomolecular condensate

The paper shows that slow diffusion in biomolecular condensates limits phosphorylation kinetics, with phosphorylation rates strongly correlated to substrate diffusion coefficients.

Long-term single-molecule Ca2+ flux recordings reveal mode-switching regulation of Ca2+-ATPases

This study uses single-molecule recordings to show Ca2+-ATPases switch between active and inactive modes, revealing a new regulatory mechanism for Ca2+ homeostasis.

Screening de novo designed protein binders in unpurified lysate using flow induced dispersion analysis

This study uses FIDA to screen computationally designed protein binders in unpurified lysates, identifying high-affinity binders efficiently.

Phase separation of protein kinase A regulatory subunits is driven by similar inter- and intra-molecular interactions involving the inhibitory segment

This study investigates the phase separation of PKA RI regulatory subunits using NMR and MD simulations, revealing similar inter- and intra-molecular interactions driving this process.

Quantification of small molecule partitioning in a biomolecular condensate with 2D NMR spectroscopy

The paper demonstrates a method to quantify small molecule partitioning between biomolecular condensate phases using 2D NMR spectroscopy without isolating components.

Reactive oxygen generation by minimal copper binding peptide motifs

This study identifies a minimal histidine-rich peptide motif capable of generating reactive oxygen species through copper coordination, suggesting broader implications for intrinsically disordered proteins.

Structure of a Ca2+ bound phosphoenzyme intermediate in the inward-to-outward transition of Ca2+-ATPase 1 from Listeria monocytogenes

The paper presents a cryo-EM structure of a Ca2+-bound intermediate in the Ca2+-ATPase 1 from Listeria monocytogenes, revealing insights into the inward-to-outward transition mechanism.