Leslie A. Kirby

CD4+ T cells cross-reactive between Epstein-Barr virus EBNA1 and the central nervous system channel anoctamin-2 (ANO2) are elevated in multiple sclerosis and exacerbate experimental autoimmune encephalomyelitis in mice.

The paper develops TRIC-DISCO, a pipeline for whole-brain 3D RNA imaging in adult mice, enabling single-cell resolution of transcriptional signatures and markers without strict RNase-free conditions.

Spatial tri-omic sequencing (DBiT ARP-seq and DBiT CTRP-seq) and CODEX imaging map chromatin, RNA, and protein dynamics across mammalian brain development and lysolecithin-induced neuroinflammation.

This study shows OPCs can cross-present antigen and mediate cytotoxicity in MS, suggesting they are co-opted by the immune system.