Gonçalo Castelo‐Branco

This paper identifies FABP8/PMP2 as a defining feature of human and old-world monkey CNS myelin, revealing molecular heterogeneity in myelin sheaths and its role in cholesterol enrichment.

KaroSpace provides a framework for interactive exploration of multi-sample spatial omics data, overcoming computational bottlenecks and proprietary software dependencies.

The paper shows that oligodendrocyte precursor cells (OPCs) use activity-dependent vesicular release to stabilize retinal ganglion cell pre-synapses in zebrafish.

CD4+ T cells cross-reactive between Epstein-Barr virus EBNA1 and the central nervous system channel anoctamin-2 (ANO2) are elevated in multiple sclerosis and exacerbate experimental autoimmune encephalomyelitis in mice.

This study shows GABAB receptors have dual roles in oligodendrocyte function and immune modulation in experimental MS, with baclofen showing therapeutic potential in reducing disease severity and promoting remyelination.

The paper performs multi-omics analysis of choroid plexus across development in mice and rats, identifying cellular remodeling, immune gene activation, and a novel epithelial subtype.

The paper develops a multi-omics guided differentiation protocol to generate PD-vulnerable midbrain dopaminergic neurons, showing improved yield and functional recovery in mice.

This study uses G4 scCUT&Tag to profile G-quadruplexes in mouse brain cells, showing lineage-specific G4s and enabling integrative analysis with scRNA-Seq data.

The paper uses single nuclei RNAseq to identify distinct glial gene expression patterns in MS patients, revealing subgroup-specific pathological processes and suggesting precision medicine approaches.

The paper develops TRIC-DISCO, a pipeline for whole-brain 3D RNA imaging in adult mice, enabling single-cell resolution of transcriptional signatures and markers without strict RNase-free conditions.

This study identifies TAF1-dependent transcriptional dysregulation as a mechanism underlying multiple sclerosis progression and presents an in vivo model for therapeutic development.

The paper shows oligodendrocytes contribute to amyloid-β plaques in Alzheimer's disease, but neurons are required for plaque formation.

Spatial tri-omic sequencing (DBiT ARP-seq and DBiT CTRP-seq) and CODEX imaging map chromatin, RNA, and protein dynamics across mammalian brain development and lysolecithin-induced neuroinflammation.

The paper profiles histone modifications in adult human CNS cells, revealing epigenetic memory of developmental programs in oligodendroglia.

The paper introduces NicheCompass, a graph deep learning method for characterizing cell niches in spatial omics data, showing improved performance in identifying and analyzing niches across multiple biological contexts.

The paper identifies regional glial heterogeneity in human white matter using single nucleus RNA sequencing, revealing tissue-specific glial populations and age/sex-related gene expression differences.

The paper introduces nano-CT, a single-cell CUT&Tag method that simultaneously profiles three epigenomic modalities, and demonstrates its application in analyzing juvenile mouse brain chromatin states.

This paper introduces spatial-ATAC-seq, a method for spatially resolved chromatin accessibility profiling at the cellular level, and demonstrates its application in mouse embryos and human tonsil tissue.

This study uses in situ sequencing to map oligodendrocyte lineage progression across brain and spinal cord regions in juvenile and adult mice, revealing spatial and temporal heterogeneity.

This study identifies an early wave of oligodendrogenesis in the human forebrain at PCW 8 using single-cell RNA-Seq and ATAC-Seq, supported by spatial gene expression analysis.

This paper presents spatial epigenome profiling using CUT&Tag and microfluidic barcoding to map histone modifications at tissue scale and single-cell level in mouse embryos and olfactory bulbs.

The paper uses single-cell Cut&Tag to profile histone modifications in the mouse brain, revealing cell identity and regulatory principles at single-cell resolution.

This study identifies Gsta4 as a regulator of apoptosis in adult oligodendrocyte differentiation and remyelination, showing its role in modulating the Fas/Casp8/Bid mitochondrial axis.

This study identifies a primed chromatin state in oligodendroglia that facilitates rapid activation of immune gene programs in multiple sclerosis.

The paper investigates cell-type specific 3D chromatin structures in the brain using immunoGAM, finding TAD melting, neuron-specific contacts, and compartmentalization patterns related to gene expression and neurodegenerative diseases.

RADICL-seq maps genome-wide RNA-chromatin interactions in intact nuclei, revealing general and cell type-specific patterns of transcript occupancy and the role of transcription in chromatin structure.

The paper identifies two functionally distinct subgroups of OPCs in zebrafish spinal cord, showing activity-dependent proliferation and differentiation.

The paper identifies distinct oligodendrocyte populations with spatial preferences and differential responses to spinal cord injury.

The paper identifies RNA binding proteins and RNAs interacting with Sox2 in mouse embryonic stem cells, showing coexistence in a ribonucleoprotein complex without evidence of chromatin recruitment regulation.