Author
Institute of Medical Science, The University of TokyoORCID 0000-0003-4780-843XUnclaimed ORCID profile · follow to get notified of new preprints
3 papers
Recent·Most cited
Riko Miyazaki, Kazuki Aibara, Haruhi Isse…·Takashi Okada
This paper develops a miRNA detection system combining TWJ-SDA, Multistep L-TEAM, and CRISPR-Cas3, achieving high sensitivity for low-concentration miRNA detection.
Kazuto Yoshimi, Kohei Takeshita, Noriyuki Kodera…·Tomoji Mashimo
This study elucidates the mechanism of CRISPR-Cas3-mediated DNA degradation in E. coli, showing how EcoCas3 cleaves ssDNA and degrades dsDNA through specific and non-specific interactions with EcoCascade.
Channabasavaiah B. Gurumurthy, Aidan R. O’Brien, Rolen M. Quadros…·Atsushi Yoshiki
The paper re-evaluates the efficiency of a CRISPR-Cas9 method for generating conditional knockout alleles in mice, finding very low success rates and high rates of undesired alleles.