This study shows DNASE1L3 clears surface DNA on B and T cells, and variants in DNASE1L3 are linked to higher surface DNA levels in autoimmune disease.

bioRxiv · Oct 5, 2025 · PreprintCell Biology

StCEL-based platform enables detection and sequencing of native sialoglycoRNA

Guang-Dong Zeng, Yunlong Wang, Xuhui Chen, Yi Ma, Zhu Zhu, Huan-Lei Liu, Liyuan Fan, Hao Nan, Ziwaregul Nur, Min Wei, Xinyuan Liu, Li Yi, Jiu-Yu Jin, Yu-Song Cao, Meng-Bo Qiu, , Xinjuan Fan

The paper introduces StCEL, a method for detecting and sequencing native sialoglycoRNA, showing its upregulation in cancers and correlation with poor prognosis.

bioRxiv · Aug 6, 2025 · PreprintMolecular Biology

Development and application of GlycanDIA workflow for glycomic analysis

Yixuan Xie, Xingyu Liu, Li Yi, Shunyang Wang, Zongtao Lin, Chenfeng Zhao, Siyu Chen, Faith M. Robison, Benson M. George, Carlito B. Lebrilla, Ryan A. Flynn, Benjamin A. Garcia

The paper develops GlycanDIA, a DIA-based workflow for glycan identification and quantification, showing improved sensitivity and the ability to detect low-abundant modified glycans.

Nature Communications · Aug 1, 2025Peer-reviewedBiochemistry

Metabolic Control of Glycosylation Forms for Establishing Glycan-Dependent Protein Interaction Networks

Xingyu Liu, Li Yi, Zongtao Lin, Siyu Chen, Shunyang Wang, Ying Sheng, Carlito B. Lebrilla, Benjamin A. Garcia, Yixuan Xie

The paper introduces GAP-MS to study glycan-dependent PPIs and shows it identifies PPI variations under different glycosylation conditions.

Proceedings of the National Academy of Sciences · Jun 18, 2025Peer-reviewedBiochemistry