Yanping Li

RAFTER quantifies mRNA translation by labeling efficiently translated RNAs with an RNA editing enzyme and releasing factor, showing correlation with Ribo-seq and Polysome-seq but with a simpler workflow enabling single-cell analysis.

This study investigates how the order of early mutations affects tumor initiation in colorectal cancer using engineered murine organoids, finding that mutation order influences tumorigenic properties and immune interactions.

The paper introduces SPARP-seq, a method combining poly(A) assessment and ribosome profiling to show poly(A) tail shortening is a hallmark of active translation in human cells.