Wei Chen

RAFTER quantifies mRNA translation by labeling efficiently translated RNAs with an RNA editing enzyme and releasing factor, showing correlation with Ribo-seq and Polysome-seq but with a simpler workflow enabling single-cell analysis.

This paper introduces DNA-based ForceChrono probes to measure single-molecule force dynamics in living cells, revealing precise loading rates and a link between force magnitude and duration.

The paper constructs a 3D spatiotemporal multi-omics atlas of Drosophila development, revealing gene regulatory networks and cell type dynamics across embryogenesis to metamorphosis.

This study uses Stereo-seq to generate high-resolution spatiotemporal transcriptomic maps of Drosophila embryos and larvae, revealing new subregions and gene regulatory networks.

DeeReCT-TSS improves TSS annotation across cell types using DNA sequences and RNA-seq data, outperforming sequence-only methods and enabling cell-type-specific TSS identification.

CASB is a sample barcoding method for single-cell sequencing that enables accurate multiplexing across different sequencing techniques and detects doublets with high sensitivity.

DeeReCT-APA quantitatively predicts alternative polyadenylation site usage across genes using a deep learning model, outperforming existing methods in multiple tasks.

This study develops a protoplasting-free method for single-nucleus RNA profiling in plants using long-read sequencing to improve cell type identification through isoform information.

The paper identifies reduced social-related neuronal activity in the retrosplenial granular cortex (RSG) of Fmr1-KO mice, leading to social novelty deficits and suggests RSG manipulation as a potential therapeutic strategy.

This study identifies Rbm10 as a splicing factor that facilitates heterochromatin assembly through its interaction with the Clr6 HDAC complex in fission yeast.

CIGAR-seq identifies mRNA modification regulators through CRISPR screening and RNA modification readout, revealing NSUN6 as a novel m5C methyltransferase.

TransMPRA combines CRISPR perturbation and MPRAs to identify trans-acting factors influencing enhancer activity.

This study identifies M segment RNA splicing as a determinant of host range in influenza A virus by comparing human- and bird-adapted strains.

This paper introduces TEPIC, a method that combines open-chromatin data with TF binding affinities to predict gene expression, achieving results comparable to TF ChIP-seq.