Liang Fang

The paper integrates mRNA and protein isoform data in human RPE-1 cells to reveal widespread translation of alternative transcripts and proteolytic processing as a major source of proteome complexity.

RAFTER quantifies mRNA translation by labeling efficiently translated RNAs with an RNA editing enzyme and releasing factor, showing correlation with Ribo-seq and Polysome-seq but with a simpler workflow enabling single-cell analysis.

This study investigates how the order of early mutations affects tumor initiation in colorectal cancer using engineered murine organoids, finding that mutation order influences tumorigenic properties and immune interactions.

PB-scope uses deep learning to analyze PB dynamics and identifies drugs affecting JAK signaling pathway.

CASB is a sample barcoding method for single-cell sequencing that enables accurate multiplexing across different sequencing techniques and detects doublets with high sensitivity.

This study develops a protoplasting-free method for single-nucleus RNA profiling in plants using long-read sequencing to improve cell type identification through isoform information.

CIGAR-seq identifies mRNA modification regulators through CRISPR screening and RNA modification readout, revealing NSUN6 as a novel m5C methyltransferase.

This study identifies a feedforward circuit between WSB1 and c-Myc that promotes cancer development through β-catenin signaling, suggesting a potential therapeutic target.