Author

Berta Escude Velasco

Broad Institute

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Genomics· 3 Neuroscience· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 4 Assay:CRISPR screen· 3 Organism:mouse· 2 Research Focus:benchmarking· 1 Research Focus:cell type annotation· 1 Research Focus:cell-cell communication· 1 Research Focus:differential expression· 1 Research Focus:drug response / drug discovery· 1

Frequent Co-Authors

John G. DoenchiD· 3 shared Alberto Cruz‐MartíniD· 2 shared Alison BrackiD· 2 shared Charlotte Yeung· 2 shared Connor JohnsoniD· 2 shared Laura M. DrepanosiD· 2 shared Luke A. FournieriD· 2 shared Rhushikesh A. PhadkeiD· 2 shared

Activity by Year

2022-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 19 mutual co-author links

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3 shared papersGenomics

John G. DoenchORCID

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Author profile →

5 papers

Recent·Most cited

Direct comparison of CRISPR knockout and interference with Perturb-seq

Laura M. Drepanos, Berta Escude Velasco, Abigail Chase, Smriti Srikanth, Michael Gatzen, Hannah Rickner, Dan Dubinsky, Andrew W. Navia, Tsukasa Shibue, Kathleen B. Yates, Peter S. Winter, John G. Doench

This study compares CRISPR knockout and interference using Perturb-seq, finding consistent transcriptional signatures and tradeoffs in off-target activity and cellular response artifacts.

bioRxiv · Jul 4, 2026 · PreprintGenomics

Balancing off-target and on-target considerations for optimized Cas9 CRISPR knockout library design

Laura M. Drepanos, Smriti Srikanth, Eleanor G. Kaplan, Spencer T. Shah, Berta Escude Velasco, Sarra Merzouk, John G. Doench

The paper presents a guide selection strategy to optimize Cas9 CRISPR knockout library design by balancing on-target and off-target activity, resulting in an updated validated library for the human genome.

Cell Genomics · Mar 25, 2026Peer-reviewedGenomics

Modular vector assembly enables rapid assessment of emerging CRISPR technologies

Abby V. McGee, Yanjing V. Liu, Audrey L Griffith, Zsofia M Szegletes, Bronte Wen, Carolyn Kraus, Nathan W. Miller, Ryan J. Steger, Berta Escude Velasco, Justin A. Bosch, Jonathan D. Zirin, Raghuvir Viswanatha, Erik J. Sontheimer, Amy B. Goodale, Matthew A. Greene, , John G. Doench

The paper introduces a modular CRISPR vector assembly system called Fragmid, enabling rapid creation and testing of CRISPR technologies through combinatorial design and pooled screening.

Cell Genomics · Mar 1, 2024Peer-reviewedGenomics

C4 induces pathological synaptic loss by impairing AMPAR trafficking.

Rhushikesh A. Phadke, Ezra Kruzich, Luke A. Fournier, Alison Brack, Mingqi Sha, Ines Picard, Connor Johnson, Dimitri Stroumbakis, Maria Salgado, Charlotte Yeung, Berta Escude Velasco, Yen Yu Liu, Alberto Cruz‐Martín

This study shows that C4 overexpression causes synaptic loss by impairing GluR1 trafficking via SNX27, independent of the complement pathway.

bioRxiv · Sep 9, 2023 · PreprintNeuroscience

Distinct VIP interneurons in the cingulate cortex encode anxiogenic and social stimuli

Connor Johnson, Lisa N. Kretsge, William Yen, Balaji Sriram, Alexandra O’Connor, Ruichen Sky Liu, Jessica Jimenez, Rhushikesh A. Phadke, Kelly K. Wingfield, Charlotte Yeung, Tushare Jinadasa, Thanh P. H. Nguyen, Eun Seon Cho, Erelle Fuchs, Eli D. Spevack, , Alberto Cruz‐Martín

The paper identifies distinct VIP interneuron subpopulations in the ACC that selectively encode anxiogenic, anxiolytic, social, or non-social stimuli in mice.

Molecular Psychiatry · Mar 4, 2022Peer-reviewedNeuroscience