Roberts Kārkliņš

Repurposing base editors for targeted knock-in and simultaneous knockouts to generate multiplex-edited allogeneic CAR T cells with minimal translocations

This paper develops BEKI, a base editor-based method for targeted transgene insertion and multiplex gene disruption in T cells, reducing chromosomal translocations compared to Cas9-based approaches.

Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases

This paper introduces one-pot PASTA, a non-viral method for efficient large transgene integration in human T cells using CRISPR and integrases, showing improved performance over existing methods.