Alessa R. Ringel

Evolution and mechanism of MEIS2-mediated forelimb specialization in bats

This study uses deep learning and functional genomics to show that MEIS2 and TWIST1 co-regulation, along with changes in regulatory regions of PBX1, underlie bat forelimb specialization.

Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases

This paper introduces one-pot PASTA, a non-viral method for efficient large transgene integration in human T cells using CRISPR and integrases, showing improved performance over existing methods.

Comparative single-cell analyses reveal evolutionary repurposing of a conserved gene program in bat wing development

The paper compares bat and mouse limb development using single-cell analyses to show how a conserved gene program is repurposed in bat wing formation.

Reconfiguration of genome-lamina interactions marks the commissioning of limb cell-fates

The paper shows that genome-lamina interactions are reconfigured during limb development to prime regulatory domains for gene activation and support cell-fate decisions.

Temporal misexpression of En1 during limb development causes distinct phenotypes

The study identifies two enhancer elements that maintain En1 expression during later limb development and shows that misexpression timing leads to distinct phenotypes.

Single cell, whole embryo phenotyping of pleiotropic disorders of mammalian development

The paper uses single-cell RNA sequencing to systematically phenotype mouse genetic models, identifying molecular and cellular changes across multiple mutants and wildtypes.

Promoter repression and 3D-restructuring resolves divergent developmental gene expression in TADs

The paper shows that divergent gene expression within TADs is resolved through TAD restructuring and DNA methylation, not enhancer-promoter compatibility or position.