William B. Black

The paper reprograms the Rossmann fold motif to create NMN+-specific enzymes that avoid NAD(P)/H interactions, achieving high specificity and productivity in biotransformation.

The paper identifies a conserved sequence motif that enables natural enzymes to use non-canonical redox cofactors, demonstrating high activity with NMN+ and other synthetic cofactors.

This paper demonstrates an activation-free method to convert carboxylic acids to aldehydes and alcohols using aldehyde dehydrogenases, achieving high yields and carbon efficiency.

This paper introduces Nox Ortho, an enzyme that enables precise control of redox reactions using NMN+ as a cofactor, achieving high specificity and enabling chiral-pure product synthesis.

This paper engineers Escherichia coli to biosynthesize nicotinamide mononucleotide (NMN+) for use in cell-free biosynthesis, achieving a 130-fold increase in intracellular NMN+ levels.